18 Acid Fast and Endospore Staining Assignment
Emilie Miller, Ph.D
Name:____________
Review Questions
- Distinguish between the Ziehl-Neelson and Kinyoun methods of acid-fast staining.
- Why is it important to leave the malachite green on the slide for at least 10 minutes in the endospore- staining procedure?
- Do you think you would find more endospores in a freshly prepared culture or in an older culture of Bacillus subtilis? Explain.
- Why do you think an infection caused by an endospore former might be harder to treat than one caused by a non-spore former?
