16 Streaking for Isolation Assignment
Emilie Miller, Ph.D
Name ______________________
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Sample source |
Area (1, 2 or 3) where isolated colonies are found |
Number of different looking colonies |
Is there evidence of contamination? |
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Your T-streak Evaluation
isolated colonies at least three
streak pattern: correct order, correct direction
amount crossovers (not too little, not too much)
entire surface used
no gouging of the agar
no inappropriate crossovers
90o angle of streaks
adequate room for final area
growth pattern: a lot to a little
label : all components, legible, on bottom of plate
incubated upside-down
Post Lab Questions
- What streaking technique is used here?
A gross lab sink is sampled with a cotton swab and plated onto this TSA plate. What streaking technique is used here?
Was the technique a good choice? Why or why not?
A pure culture is streaked onto this TSA plate. What technique is used here?
Was the technique a good choice?
What did the person likely do wrong when performing the technique? (There are at least 2 things.)
What technique is used on this plate?
Was the technique a good choice?
What did the person likely do wrong when performing the technique?
