14 Aseptic Transfer Assignment
Emilie Miller, Ph.D
Data and Observations
Name_______________
|
Organism |
Medium form |
Description of growth before mixing |
Description of growth after mixing |
Relative amount of growth 0-5 (0=none, 5=very turbid) |
|
Staphylococcus epidermidis from broth* |
*to broth |
|
|
|
|
S. epidermidis from broth* |
*to slant |
|
n/a |
|
|
S. epidermidis from plate* |
*to broth |
|
|
|
|
S. epidermidis from plate* |
*to slant |
|
n/a |
|
Post Lab Questions
1. If someone had growth in the practice tubes labeled “sterile,” what does that indicate?
2. Draw the growth in one of your slant tubes the figure on the right, or freehand.
3. What inoculation technique did you use?
4. What instrument did you use?
5. Think about the relative amount of growth for each tube in terms of the initial amount of inoculum. If the inoculum came from growth on solid medium, there may have been more cells compared to an innoculum from a broth culture. Please describe and explain any correlation that you notice.
6. Notice how the scientific name for the organism is written in the data table. Describe the format the first time the name is written. (Use these terms: genus, capital, lower case) In all future lab reports, you will be required to use this format.
7. Explain why disinfection and sterilization are not interchangeable.