16 Streaking for Isolation Assignment

Name ______________________

Your T-streak Evaluation T-Streak

isolated colonies at least three

streak pattern: correct order, correct direction

amount crossovers (not too little, not too much)

entire surface used

no gouging of the agar

no inappropriate crossovers

  90^o angle of streaks

adequate room for final area

growth pattern: a lot to a little

label : all components, legible, on bottom of plate

incubated upside-down

Post Lab Questions

• What streaking technique is used here?

• A gross lab sink is sampled with a cotton swab and plated onto this TSA plate. What streaking technique is used here?

Was the technique a good choice? Why or why not?

• A pure culture is streaked onto this TSA plate. What technique is used here?

Was the technique a good choice?

What did the person likely do wrong when performing the technique? (There are at least 2 things.)

• What technique is used on this plate?

Was the technique a good choice?

What did the person likely do wrong when performing the technique?